Transendothelial electrical resistance measurement by a microfluidic device for functional study of endothelial barriers in inflammatory bowel disease

Introduction: Inflammatory bowel disease (IBD) is a chronic relapsing and remitting with rising incidence globally. Circulating exosomes play great roles in IBD pathogenesis through exosomal cargoes, especially impacting the function of endothelial barriers. Transendothelial electrical resistance (TEER) measurement widely used non-invasive label-free strategy to monitor barrier vitro . This study established well-designed microfluidic device TEER changes cellular on-chip after treated exosome derived from serum. Methods: The chip comprised two layers chambers top layer for perfusion medium maintain nutrition pressure during cell culture, bottom extracellular matrix mimic using hydrogel, which are separated by semipermeable membrane that permitted formation barrier. Four electrodes independent outlets were integrated detection. In vivo mouse models proteome profiling performed finding relevant regulators. Results: With this platform, significant decrease was detected, indicating serum impact on-chip. models, group showed higher DAI scores, shorter colons, more severe histological features, levers S100A8 expression, promoting progress. Proteome TFRC ANXA5 have potentials as novel regulators IBD. Discussion: in-house customized emulates microenvironment enables monitoring, can be investigate promote severity disease.